Figure 3.

miR‐31a‐5p regulates the downstream expression of genes in the SATB2 pathway. (a) miR‐31a‐5p mimics remarkably decreased the number of mineral deposits of BMSCs. (b and c) In transfected cells, Western blot and qRT‐PCR showed that the expression of SATB2 and the osteogenic markers, OSX and OCN, were also reduced by miR‐31a‐5p mimics. (d) Inhibition of miR‐31a‐5p dramatically increased mineral deposits and (e and f) enhanced osteogenic markers. (g) SATB2 was predicted as the target of miR‐31a‐5p by TargetScan. (h) Western blot showed the SATB2 expression and (i) Alizarin red staining reveals that inhibition of miR‐31a‐5p rejuvenated the compromised osteogenic capacity of si‐SATB2 treated BMSCs. ^# p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. Data are presented as the mean ± SD, n = 3