Figure 1.

Schematic sketch of the immunomagnetic separation-based microvesicle-dependent plasmin generation assay.

A. Microvesicles (MVs) were extracted from platelet-free plasma (PFP) using magnetic beads coated with specific antibodies. B. Plasminogen was added, and its cleavage to plasmin was activated by urokinase present on the MV surface during incubation at 37°C. C. The generated plasmin was quantified by colorimetry using a specific chromogenic substrate (CBS0065, Stago), and the optical density (OD) at 405 nm was measured. IMS = immunomagnetic separation.