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. 2018 Jul 17;7(1):1490144. doi: 10.1080/20013078.2018.1490144

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Characteristics of extracellular vesicles released by GSCs vary as a function of subtype and differentiation. (a) NTA analysis of EVs size distribution indicates that differentiated MES cells (MES DIFF) exhibit a significantly increased release of EVs smaller than 150 nm. (b) NTA profiles of the total particle numbers released by the GSC cell populations. Differentiation significantly increases the number of particles released by these glioma cells. (c) Cryo-TEM of EVs isolated from GSC and their differentiated counterparts illustrating the heterogeneity in EV size and CD63 immunogold labelling (dark particles). The images represent PN 10K pellet (left and middle panels) and MES DIFF 100K fraction (right panel), additional images are shown in Figure S3.