Extended Data Figure 8. The integrated analysis agrees with an independent tSNE analysis of each timepoint.

A) tSNE visualizations of interneuron precursors from E13.5, E18.5, and P10, calculated independently for each timepoint. Cells are colored as in Figure 4B-D, based on their mapping to P56 datasets in integrated analysis. However, since the tSNE was performed separately for each timepoint, we can assess how the integrated analysis agrees with an independent analysis of each timepoint. In each case, we can see that the ‘cardinal type’ separation we observe via integrated analysis (Figure 4B-D) is consistent with an independent analysis of each dataset. Integrated analysis with the P56 dataset results in clearer separation, and enables us to map developmental precursors to adult subtypes.

B) Expression of Gad1 and Meis2 in single cell datasets. Cells expressing both genes are likely projection neuron precursors that have recently been described in the CGE²¹, but whose progeny is not captured in the mouse visual cortex dataset. Therefore, these cells are correctly mapped as ‘unassigned’.