Figure 5.

SIRT4 overexpression induces spindle defects and chromosome misalignment in oocyte meiosis. (A) Control (a) and SIRT4‐OE (b–d) oocytes were stained with α‐tubulin antibody to visualize the spindle (green) and counterstained with PI (red) to observe chromosomes. Arrows indicate spindle defects and arrowheads indicate chromosome misalignment in MII oocytes. (B) Quantification of control and SIRT4‐OE oocytes with spindle/chromosome defects. Data are expressed as mean percentage ± SD from three independent experiments in which at least 120 oocytes were analyzed. *p < 0.05 vs. controls. Scale bars: 20 μm