Figure 3.

NMJ morphology in BRASTO mice and age‐matched controls. NMJ morphology in young (7‐month‐old) BRASTO mice (b, d) did not differ from characteristic, healthy NMJ morphology seen in age‐matched controls (a, c). Aged (25‐month‐old) BRASTO (f, h) mice have a more youthful NMJ morphology than age‐matched control (Ctr) mice (e, g). NMJs in BRASTO mice have distinct tSCs, clear innervation (arrow), and pretzel‐shaped motor endplates (arrowhead in f, h) in contrast to the fewer tSCs, incomplete or absent innervation, and fragmented AChRs (arrowheads in e, g) of NMJs in Ctr mice. Representative images of NMJs from sternomastoid muscle stained with S100 Ab (for tSCs, green), NF200 Ab (for neurofilaments, green), BTX (for AChRs, red), and DAPI (nuclear staining, blue). Scale bar = 20 μm