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. 2018 Jun 19;17(4):e12792. doi: 10.1111/acel.12792

Figure 6.

Figure 6

Inhibitory effects of L5 on telomerase expression and activity in human aortic endothelial cells (HAECs). HAECs were treated with phosphate‐buffered saline (PBS) (control), 50 μg/ml L1, or 50 μg/ml L5. (a) Representative western blot analysis showing hTERT protein expression in treated and control cells. Quantification of the results is shown (n = 3 independent experiments per group). **p < 0.02 vs. control. (b) Immunofluorescence staining of hTERT (green) in treated and control cells. DAPI was used as a nuclear counterstain (blue). (c) Telomerase activity of cells treated with PBS, 50 or 100 μg/ml L1, or 50 or 100 μg/ml L5. n = 3 independent experiments per group. **p < 0.02 and ***p < 0.01 vs. control. (d) Representative western blot analysis after experiments with siRNA against TP53 (siRNA TP53) and control siRNA (nontargeting siRNA, siRNA NT). TP53 is required for the L5‐induced downregulation of hTERT expression in HAECs. (e) Quantification of the western blot results shown in (d) from three independent experiments. *p < 0.05 vs. nontargeting siRNA (siRNA NT)