Figure 2.
The effects of TUDCA on ROS, LDH, GSH, and SOD in H2O2-induced changes in NRCMs. A, The ROS levels indicated by the intensity of DCFH fluorescence in NRCMs. Rosup was a compound mixture in the ROS assay kit which was used as positive control in inducing ROS generation. B, The LDH release from NRCMs. C, Intracellular GSH level of NRCMs. D, Intracellular SOD level of NRCMs. Data are presented as mean ± SEM. n = 3, *P < .05, **P < .01 compared to control. #P < .05, ##P < .01, ###P < .001. GSH indicates glutathione; LDH, lactic dehydrogenase; NRCM, Neonatal rat cardiomyocytes; ROS, reactive oxygen species; SEM, standard error of the mean; SOD, superoxide dismutase; TUDCA, tauroursodeoxycholic acid; DCFH, dichloro-dihydro-fluorescein.