Table 1.
Antibodies used in this study.
| Name | Epitope | Type | Origin | Provider | Dilution |
|---|---|---|---|---|---|
| Anti-A2AR | CTER 33aa | Poly | Guinea Pig | Frontier Institute | 1/200 |
| Anti-GFAP | GFAP | Poly | Rabbit | Dako | 1/1000 |
| Anti-NeuN | NeuN | Mono | Mouse | Merck Mllipore | 1/500 (IHC) 1/1000 (WB) |
| Anti-Aβ 1-16 (6E10) | Total Aβ (3-8 aa) | Mono | Mouse | Biolegend | 1/1000 |
| Anti-APP (C17) | Cter part of APP, CTFs | Poly | Rabbit | Home Made | 1/5000 |
| Anti-GluR1 | Hulan GluR1 (840–850 aa) | Mono | Rabbit | Merck Mllipore | 1/2000 |
| Anti-phospho-GluRl (Ser831) | pSer831 | Mono | Rabbit | Merck Mllipore | 1/1000 |
| Anti-phospho-GluR1 (Ser845) | pSer845 | Mono | Rabbit | Merck Millipore | 1/1000 |
| Anti-GluR2 | Mouse GluR2 (150–250 aa) | Poly | Rabbit | Abcam | 1/5000 |
| Anti-phospho-GluR2 (Ser88O) | pSer88O | Poly | Rabbit | Abcam | 1/5000 |
| Anti-NR2B | Mature NR2B (1437–1456 aa) | Poly | Rabbit | Cell Signaling | 1/1000 |
| Anti-phospho-NR2B (Tyr1472) | pTyr1472 | Poly | Rabbit | Cell Signaling | 1/1000 |
| Anti-phospho-NR2B (Tyr1480) | pTyrl480 | Poly | Rabbit | Thermo Fisher Scientific | 1/1000 |
| Anti-Munc-l8-1 (Muncl8) | Cter (577–594 aa) | Poly | Rabbit | Sigma | 1/2000 |
| Anti-PSD95 | PSD95 | Poly | Rabbit | Cell Signaling | 1/1000 |
| Anti-Spinophiline | Spinophiline | Poly | Rabbit | Merck Mllipore | 1/1000 |
| GAPDH | Hutnan GAPDH (FL 1–335) | Poly | Rabbit | Santa Cruz Biotechnology | 1/1000 |
Abbreviations: Mono, monoclonal; Poly, polyclonal; IHC, dilution used in Immunohistochemistry; WB, dilution used in Western blotting; GFAP, glial fibrillary acidic protein; NeuN, neuronal nuclear; APP, amyloid precursor protein; GAPDH, glyceraldehyde-3-phosphate.