Fig. 4.

Graphical sequence logo representing the sequence patterns of RING, U-box and F-box domain in identified corresponding type of soybean E3 proteins. Weblogos are generated based on the multiple sequence alignment of RING (a), U-box (b) and F-box (c) domains. Red and blue arrowheads in (a) indicate the zinc binding residues of RING domains and the conserved hydrophobic residues that interact with cognate E2s. Red arrowheads in (b) indicate the hydrophobic E2-binding residues whereas blue and light blue ones indicate conserved residues in two cores of hydrophobic interactions. Red and blue arrowheads in (c) mark the conserved residues that contribute to the formation of α1-helix and the packing of the F-box helices whereas residues marked with star sign are the positions important for human Skp2 contacting with Skp1