Table 1.
Primers used in polymerase chain reaction and quantitative real-time polymerase chain reaction for gene expression analysis
| Gene | Primer sequence (5′to3′) |
|---|---|
| CD29-F | GTCACCAACCGTAGCAA |
| CD29-R | CTCCTCATCTCATTCATCAG |
| CD44-F | CGATTTGAATATAACCTGCCGC |
| CD44-R | CGTGCCCTTCTATGAACCCA |
| CD166-F | GGACAGCCCGAAGGAATACGAA |
| CD166-R | GACACAGGCAGGGAATCACCAA |
| CD4-F | GATGGAGGTGGAACTGC |
| CD4-R | GGAAAGCCCAACACTATG |
| CD8-F | GGGTGGAAAAGGAGAAGC |
| CD8-R | AGGTGAGTGCGGGAGAC |
| CD14-F | CAGGTGCCTAAGGGACT |
| CD14-R | AATAAAGTGGGAAGCGG |
| Coll II-F | CAGGATGTCCAGGAGGCT |
| Coll II-R | GCAGTGGCGAGGTCAGTAG |
| AGC-F | GGAGCCCGAGCCTATACTATTT |
| AGC-R | CCCAAGGACCACCAATCA |
| IL-6-F | AGGGAGGTCGAGCTGTTCTC |
| IL-6-R | GGAGTGTTCACTAAGCGGTCA |
| IL-1β-F | CGGTCAAGGAGAGGAGCTTAC |
| IL-1β-R | GGACTAGCCCTCGCTTATCTTT |
| TNF-α-F | GGAGAAGCCGGTAGTGGAGAT |
| TNF-α-R | GGTCTGGTCACGGTTTGGAA |
| IL-8-F | CACAGTGGACGACATCCGAAA |
| IL-8-R | AGCTACATAGGAATTACGGGCAA |
| MMP-1-F | CGACTCGCTATCTCCAAGTGA |
| MMP-1-R | GTTGAACCAGTCTCCGACCA |
| MMP-13-F | GGAGGCGAGAACATCAAGCC |
| MMP-13-R | CGGCCTTCCCTCGTAGTGA |
| OCT4-F | ACCTTCATCGGAAACTCCAAAG |
| OCT4-R | ACTGTTAGGCTCAGGTGAACT |
| Nanog-F | CTGTGGGTTTCTGTGCTGG |
| Nanog-R | CCGGCTTCAAGGCTTTCAG |
| GAPDH-F | ACTTTGTGAAGCTCATTTCCTGGTA |
| GAPDH-R | GTGGTTTGAGGGCTCTTACTCCTT |