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. 2018 Jul 18;49:69. doi: 10.1186/s13567-018-0567-1

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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DNA fragmentation of M. bovis-infected macrophages was not inhibited by 3-ABA treatment. Bovine macrophages were pretreated with 50 µM of 3-ABA before M. bovis infection with AN5 strain (10:1) during 16 h. Quantification of DNA fragmentation shows mean fluorescence intensity (MFI) fold change of M. bovis-infected and staurosporine-treated (0.5 µM) macrophages with respect to uninfected cells. The results are representative of three independent experiments. Asterisk represent a p value equal or less than 0.05 of statistical significance.