Table 1.
Composition, EE%, DL% and physico-chemical properties of rutin-loaded Eudragit S100 nanospheres (n = 3; data are expressed as the mean ± SD).
| Formulae code | Drug: polymer (weight ratio)* | Poloxamer-188 (w/v%) | EE% | DL% | Particle size (nm)** | PDI | Zeta potential (mV) |
|---|---|---|---|---|---|---|---|
| F1 | 1:1 | 0.25% | 97.99 ± 0.05 | 49.49 ± 0.01 | 154.90 ± 44.40a,b | 0.31 ± 0.09 | −21.80 ± 4.06 |
| F2 | 1:1 | 0.50% | 98.10 ± 0.50 | 49.52 ± 0.13 | 130.30 ± 35.29a | 0.29 ± 0.10 | −22.90 ± 5.18 |
| F3 | 1:1 | 0.75% | 97.40 ± 0.10 | 49.34 ± 0.02 | 141.30 ± 48.81a | 0.31 ± 0.15 | −25.20 ± 4.29 |
| F4 | 1:2 | 0.25% | 96.23 ± 0.84 | 32.46 ± 0.19 | 207.60 ± 56.60a,b | 0.48 ± 0.01 | −21.70 ± 4.78 |
| F5 | 1:2 | 0.50% | 96.33 ± 0.66 | 32.49 ± 0.15 | 190 ± 46.60a,b | 0.47 ± 0.15 | −22.10 ± 4.38 |
| F6 | 1:2 | 0.75% | 95.85 ± 0.45 | 32.38 ± 0.10 | 350.80 ± 73.17b | 0.46 ± 0.01 | −20.50 ± 4.78 |
| F7 | 1:4 | 0.25% | 94.19 ± 0.33 | 19.06 ± 0.05 | 850 ± 205.70c,d | 0.42 ± 0.11 | −26.70 ± 5.59 |
| F8 | 1:4 | 0.50% | 94.32 ± 0.14 | 19.08 ± 0.02 | 716.20 ± 74c | 0.44 ± 0.08 | −27.30 ± 5.81 |
| F9 | 1:4 | 0.75% | 94.80 ± 0.71 | 19.16 ± 0.11 | 968.6 ± 261.30d | 0.48 ± 0.03 | −26.90 ± 6.34 |
*
The net weight of the drug and polymer is 100 mg in all the developed formulations.
**
Means assigned with the same letter are statistically non-significant different, while different letters denote a statistically significant difference between means at P < 0.05.