Fig. 1.

(A) Slices through tomograms of E. coli cells slightly over-expressing FtsZ(D212A) protein. A cross-section of FtsZ filaments (white arrows) can be seen at the E. coli membrane constriction site (upper). Filaments can be seen across the longitudinal axis at a distance of ~16 nm from the bacterial inner membrane (lower). IM = inner membrane, OM = outer membrane. Scale bars; 50 nm. Adapted from [12]. (B) Magnetosome chain growth in M. magneticum in the presence (left) and absence (right) of iron. Segmentation of the latter condition is shown (lower) highlighting magnetosomes (yellow) and their associated filaments (green) and the cellular context (blue). Arrows indicate long cytoskeletal filaments. Scale bars; 100 nm. Adapted with permission from [15]. (C) Single tomographic slices from high resolution cryo-ET reconstructions of M. xanthus, with object segmentations and overlaid cryo-PALM signal. A very short loaded T6SS structure with a ‘baseplate’ attached to the membrane (left) and a distinctive bent T6SS structure (right) are shown. Segmentations show different tubular structures (blue, green, pink) and spherical granules (white). Scale bar; 400 nm. Adapted with permission from [19].