Fig. 2. TP images of exogenous HClO with QClO in HeLa cells. HeLa cells were treated with 5.0 μM QClO for 0.5 h (a, b, and c) and then with 50 μM HClO for 0.5 h (d, e, and f). (g) Fluorescence intensity ratios from overlay images of (c) and (f). The fluorescence intensities were collected at the blue channel (400–500 nm) and the green channel (550–600 nm) respectively upon two-photon excitation at 820 nm. Scale bar: 20 μm. Incubation time: 0.5 h.
