Fig. 4. TP images of the HClO level in wounded tissues of mice with QClO. The control mice and wounded mice were injected with 150 μL of 1.0 mM QClO for 1.0 h, respectively, and the tissues were harvested and imaged. (a, e, and i) TP images of the control mice tissues. (b, f, and j) TP images of the wounded tissues of mice at day 1. (c, g, and k) TP images of the wounded tissues of mice at day 4. (d, h, and l) The wounded mice at day 4 were injected with 150 μL of 1.0 mM QClO and 5.0 mM NAC for 1.0 h, and then the tissues were harvested and imaged. (m) Fluorescence ratios (green/blue) of the ratio images (i), (j), (k) and (l). The fluorescence emissions were collected at the blue channel (a–d, 400–500 nm) and green channel (e–h, 550–600 nm) upon excitation at 820 nm. Scale bar: 50 μm.