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. 2018 Jun 27;24:4433–4439. doi: 10.12659/MSM.906838

Figure 1.

Figure 1

Effect of human TNF-α on ICAM-1 in HCAEC. (A–C) The level of ICAM was detected after treatment with different concentrations of TNF-α for indicated times. The ICAM-1 mRNA level (A), protein expression in membrane (B), and in the supernatant (C) were detected by qRT-PCR, Western Blot, and ELISA, respectively. The concentrations of TNF-α are labeled on the right. (D–F) The level of ICAM was detected after treatment with TNF-α and TNF-αR siRNA. The ICAM-1 mRNA level (D), protein expression in membrane (E), and in the supernatant (F) were detected by qRT-PCR, Western blot, and ELISA, respectively. Normal Group, cells without treatment; TNF-α, cells were treated with 2.5 μg/l TNF-α for 6 h; siRNA, cells were transfected with siRNA of TNF-α receptor; TNF-α+siRNA, cells were first transfected with siRNA of TNF-α receptor and then with 2.5 μg/l TNF-α for 6 h; * P<0.05 compared with the normal group; & P<0.05 compared with the siRNA group; # P<0.05 compared with siRNA+TNF-α group.