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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Cancer Res. 2018 May 7;78(13):3522–3531. doi: 10.1158/0008-5472.CAN-17-4049

Figure 2. Effects of miR-339-5p overexpression on cell growth and viability.

Figure 2

Q-PCR analysis demonstrates high-level miR-339-5p overexpression in transduced BBC2 cells relative to endogenous levels (A). BBC2 cells overexpressing miR-339-5p show a highly significant increase in cell growth in GFP competition assays (B) as well as cell viability using trypan blue exclusion assays (C) and luminescence viability assays (D), compared with the empty vector transduced cells. In the analysis using human KG1 cells, the same effects of miR-339-5p overexpression were seen (E–H). When BBC2 cells were treated with miR-339-5p sponges (SP), cell viability demonstrated by trypan blue exclusion (I) and luminescence assays (J) was significantly reduced. Flow cytometric analysis (K) demonstrates the sponge-treated cells show a significant increase in apoptosis. The same effects were seen for KG1 cells treated with the miR-339-5p sponges (L–N). *** p = <0.001, **** p = <0.0001.