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. 2018 Jul 12;5:160. doi: 10.3389/fvets.2018.00160

Figure 1.

Figure 1

Analysis of variability of the TgRs selected for the detection of FMDV. Entropy plots obtained from 328 sequences of FMDV for each TgR are shown, (A) TgR1, (B) TgR2, (C) TgR3, and (D) TgR4. The global entropy value estimated by the entropy media value and standard error associated to each TgR is also shown. Different letters mean statistical differences (p < 0.05). Entropy plots for each primer of each TgR are also shown (E) FP/RP-TgR1, (F) FP/RP-TgR2, (G) FP/RP-TgR3, and (H) FP/RP-TgR4; the sequences for each forward and reverse primer are denoted, critical positions for the last four nucleotides at the 3′OH of each primer are indicated with black arrows. Entropy plots for the probe region obtained from the assays reported by Reid et al. (9), Callahan et al. (10), and Moniwa et al. (33) are shown, (I) probe matching TgR1, (J) probe matching TgR2, and (K) probe matching TgR3. The global entropy value estimated by the entropy media value and standard error associated to each TgR is also shown. The sequences of each probe are denoted and critical positions (H > 0.1) for each probe are indicated with black arrows.