Table 1.
Primers used in RT-qPCR analysis
| Accession number gene bank | Gene | Primer (5′→3′) |
|---|---|---|
| NM_017008.4 | GAPDH (F) | GCTGAGTATGTCGTGGAG |
| GAPDH (R) | TCTTCTGAGTGGCAGTGAT | |
| NM_021745.1 | FXR (F) | TGAGCGTCTACAGCGAAAGTG |
| FXR (R) | GGGATGGTGGTCTTCAAATAAG | |
| NM_057133.1 | SHP (F) | ATCTCTTCTTCCGCCCTGTC |
| SHP (R) | AGGTTTTGGGAGCCATCAAG | |
| NM_012942.2 | CYP7A1 (F) | GCAAAACCTCCAATCTGTCAT |
| CYP7A1 (R) | GCTTCAAACATCACTCGGTAAC | |
| NM_031760.1 | BSEP (F) | CGTGCTTGTGGAAGAAGTTG |
| BSEP (R) | GGGAGTAGATGGGTGTGACTG | |
| AF286469 | SREBP-1c (F) | CAAGTGCTGCAGGAAACTGA |
| SREBP-1c (R) | CATGGCCTTGTCAATGGAAC | |
| NM_198780.3 | PEPCK (F) | CTGCCTCTCTCCACACCATT |
| PEPCK (R) | GCCTTCCACAAACTTCCTCA | |
| NM_013098.2 | G6Pase (F) | TCCGTGCCTCTGATAAA |
| G6Pase (R) | CCACGAAAGATAGCGAGA | |
| NM_012675.3 | TNF-α (F) | GAAAAGCAAGCAACCAGCCA |
| TNF-α (R) | CGGATCATGCTTTCCGTGCTC | |
| NM_001276711.1 | NF-κB (F) | TCTTCAACATGGCAGACGAC |
| NF-κB (R) | CTCTCTGTTTCGGTTGCTCT |
Abbreviations: F, forward primer; R, reverse primer; RT-qPCR, quantitative reverse-transcription polymerase chain reaction.