Figure 2.

Localization of the LHCX isoforms in different chloroplast fractions. A, Suc density gradient fractionation of solubilized thylakoids from wild-type (WT) and lhcx1 cells grown in LL and in HL for 2 d. FP, Free pigments; PSII m, PS II monomers; PSII d, PSII dimers. B, Western-blot analysis of the proteins extracted from the thylakoids and the FCP, PSII, and PSI fractions and detected with antibodies against LHCSR/LHCX, LHCF (antenna proteins), D2 (PSII), and PsaF (PSI). Gray images represent no signal detected after hybridization with the indicated antibodies. Samples were loaded at an equal chlorophyll amount (1 µg). CBB, Coomassie Brilliant Blue staining of the protein gels.