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. 2018 May 29;177(3):990–1006. doi: 10.1104/pp.17.01815

Figure 4.

Figure 4.

CsALDH3I1 mediates crocetin biosynthesis in vitro. A, HPLC chromatograms (absorbance at 440 nm) of in vitro assays performed for 120 min in the absence (THIO) and presence (CsALDH3I1:THIO) of CsALDH3I1. STANDARD = crocetin dialdehyde authentic standard. In the presence of CsALDH3I1, the crocetin dialdehyde is converted into crocetin (1, trans-isomer, and 2, cis-isomer; Supplemental Fig. S4). B, Time course of crocetin dialdehyde conversion in the presence of CsALDH3I1. Data are averages ± sd of three biological replicates and expressed as fold internal standard (Fold IS).