Figure 6.

Tissue specificity and solubility of enzymes involved in crocin biosynthesis. A, Immunoblot analysis performed on total proteins (10 µg) extracted from C. sativus stigmas, stamens, and tepals, performed with rabbit polyclonal antibodies raised against CsCCD2, CsALDH3I1, and CsUGT74AD1 peptides. B, Immunoblot analysis performed on total protein extract from C. sativus stigmas. Lane 1, Soluble proteins extracted with PB buffer; lane 2, extrinsic membrane proteins extracted with PB buffer + 200 mm NaCl; lane 3, insoluble proteins extracted with SDS loading buffer (for details, see “Materials and Methods”).