Figure 10.

Heterologous complementation of Atgmt1 with GINT1. A, Heterozygous Atgmt1 plants were transformed with CaMV 35S_pro:AtGMT1, 35S_pro:AtGINT1, or 35S_pro:OsGINT1. Bar = 2 cm. T2 seedlings (two independently transformed lines per construct) were analyzed by RT-PCR (B). Sphingolipidomic analyses using LC-MS/MS (C and D). C, Profiles of all the major classes of GIPCs detected in the tissues tested, and D, amounts of either Hex_n-only GIPCs (Hex) or HexN(Ac)-containing GIPCs [HexN(Ac)]. PP2AA3, PROTEIN PHOSPHATASE 2A SUBUNIT A3; −RT, reaction lacking reverse transcriptase enzyme. Data are means of three biological replicates (>50 seeds per replicate) ± sd.