GIPC composition of Atgint1 plants. A to H, LC-MS/MS was used to determine the GIPC composition of dry seeds (A and B), leaves of 3-week-old plants (C and D), flowers (E and F), and developing green siliques (G and H). A, C, E, and G, Accumulation profiles of all the major GIPC classes. B, D, F, and H, Overall amounts of either Hexn-only GIPCs (Hex) or HexN(Ac)-containing GIPCs [HexN(Ac)]. Data are means of three biological replicates ± sd. Asterisks indicate significant difference compared to that in the wild type (Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001).