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. 2018 Jan 31;84(09-10):584–593. doi: 10.1055/s-0044-100188

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This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.

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Fig. 1 — Graphic representation of the DESI-IMS workflow. a The ionization mechanism and desorption event are achieved directly at the sample surface to deliver molecular images (adapted from Takáts et al., 2004) ⁵⁶ . b Relative intensities observed for multiple ions ( m/z ) represented with different colors in various biological systems (i.e., polymicrobial culture, plant and animal, respectively). Sensitive parameters, which strongly affect the resolution include the geometry between the DESI components ( x, y, z axis), such as the distance of the sample surface to the sprayer tip ( d 1) and to the MS inlet ( d 2).