Fig 6. Dialect training requires multiple sensory inputs.

(A) Experimental design of dialect training for flies that are used as students using only visual cues (panels B,C). Flies only see each other through the duplex, with no direct interaction. Two species are co-incubated for one week prior to being used as students. Percentage of eggs laid by exposed flies normalized to eggs laid by unexposed flies is shown. Communication between trained students D. melanogaster and D. ananassae with training through visual cues only, shows that visual cues are not sufficient (B, C). Communication between trained students D. melanogaster and D. ananassae with training through monochromatic, red light only, shows a lack of dialect training (D, E). Communication between trained students ewg^NS4, mutant flies, and D. ananassae shows that moving wings are necessary (F, G). Communication between trained students Orco¹ and D. ananassae shows that olfactory cues are necessary (H, I). Communication between trained students Ir8a¹ and D. ananassae shows that Ir8a is a necessary receptor (J, K). Communication between trained students D. melanogaster and D. ananassae with training by male D. melanogaster only or by female D. melanogaster only, is not sufficient for dialect training (L,M). Error bars represent standard error (n = 12 biological replicates) (*p < 0.05).