Table 6. Microneutralization test of group C orthobunyavirus (GRCV) using hyperimmune mouse ascitic fluids (HMAFs) prepared with prototype strains.
| Year | Code | APEUV HMAF | CARV HMAF | ITQV (PE00036) HMAF | MTBV HMAF | MURV HMAF | ORIV HMAF | Complex |
|---|---|---|---|---|---|---|---|---|
| 1995 | IQT1726 | NEG | 1/80 | NEG | NEG | NEG | NEG | CARV |
| 1999 | IQU0250 | NEG | 1/40 | NEG | NEG | NEG | NEG | CARV |
| 1999 | IQU0540 | NEG | 1/320 | NEG | NEG | NEG | NEG | CARV |
| 1999 | IQU1719 | NEG | 1/80 | NEG | NEG | NEG | NEG | CARV |
| 1999 | IQU1840 | NEG | 1/40 | NEG | NEG | NEG | NEG | CARV |
| 1999 | IQU2233 | NEG | 1/80 | NEG | NEG | NEG | NEG | CARV |
| 2000 | IQU2992 | NEG | 1/40 | NEG | NEG | NEG | NEG | CARV |
| 2006 | FMD0783 | NEG | 1/80 | NEG | NEG | NEG | NEG | CARV |
| 2006 | IQE3155 | NEG | 1/80 | NEG | NEG | NEG | NEG | CARV |
| 2007 | FMD1692 | NEG | 1/80 | NEG | NEG | NEG | NEG | CARV |
| 2007 | FMD1729 | NEG | 1/40 | NEG | NEG | NEG | NEG | CARV |
| 2007 | IQE5249 | NEG | 1/80 | NEG | NEG | NEG | NEG | CARV |
| 2007 | IQE5417 | NEG | 1/80 | NEG | NEG | NEG | NEG | CARV |
| 2008 | FMD2084 | NEG | 1/40 | NEG | NEG | NEG | NEG | CARV |
| 2009 | FMD2329 | NEG | 1/80 | NEG | NEG | NEG | NEG | CARV |
| 2009 | IQE9621 | NEG | 1/160 | NEG | NEG | NEG | NEG | CARV |
| 2010 | FSL4398 | NEG | 1/80 | NEG | NEG | NEG | NEG | CARV |
| 2011 | FPI02066 | NEG | 1/80 | NEG | NEG | NEG | NEG | CARV |
| 2011 | FPI02188 | NEG | 1/80 | NEG | NEG | NEG | NEG | CARV |
| 2011 | FPI02660 | NEG | 1/80 | NEG | NEG | NEG | NEG | CARV |
| 1999 | IQU0212 | 1/20 | 1/80 | NEG | NEG | NEG | NEG | CARV-APEUV |
| 2003 | IQD5973 | 1/20 | 1/80 | NEG | NEG | NEG | NEG | CARV-APEUV |
| 2004 | FSL1813 | 1/20 | 1/160 | NEG | NEG | NEG | NEG | CARV-APEUV |
| 2008 | FMD1899 | 1/20 | 1/80 | NEG | NEG | NEG | NEG | CARV-APEUV |
| 2012 | FPI3933 | 1/320 | 1/640 | NEG | NEG | NEG | NEG | CARV-APEUV |
| 2013 | FPI5338 | 1/320 | 1/640 | NEG | NEG | NEG | NEG | CARV-APEUV |
| 2000 | FSL0167 | 1/20 | 1/320 | NEG | 1/20 | NEG | NEG | CARV-APEUV-MTBV |
| 2007 | FMD1643 | 1/20 | 1/160 | NEG | 1/20 | NEG | NEG | CARV-APEUV-MTBV |
| 2007 | FMD1684 | 1/40 | 1/160 | NEG | 1/20 | NEG | NEG | CARV-APEUV-MTBV |
| 2007 | IQE5082 | 1/40 | 1/160 | NEG | 1/20 | NEG | NEG | CARV-APEUV-MTBV |
| 2008 | FMD1881 | 1/40 | 1/160 | NEG | 1/20 | NEG | NEG | CARV-APEUV-MTBV |
| 2009 | IQE8988 | 1/20 | 1/320 | NEG | 1/20 | NEG | NEG | CARV-APEUV-MTBV |
| 2011 | FPI02530 | 1/40 | 1/160 | NEG | 1/20 | NEG | NEG | CARV-APEUV-MTBV |
| 2007 | FMD0996 | 1/40 | 1/320 | NEG | 1/40 | 1/20 | NEG | CARV-APEUV-MTBV-MURV |
| 2011 | FPI02450 | 1/80 | 1/320 | NEG | 1/40 | 1/20 | NEG | CARV-APEUV-MTBV-MURV |
| 2011 | MIS1035 | 1/40 | 1/640 | NEG | 1/40 | 1/40 | NEG | CARV-APEUV-MTBV-MURV |
| 2001 | FSL0512 | NEG | 1/160 | NEG | 1/20 | NEG | NEG | CARV-MTBV |
| 2003 | IQD6203 | NEG | 1/160 | NEG | 1/40 | NEG | NEG | CARV-MTBV |
| 2004 | FSL1270 | NEG | 1/320 | NEG | 1/40 | NEG | NEG | CARV-MTBV |
| 2011 | FPI02289 | NEG | 1/160 | NEG | 1/20 | NEG | NEG | CARV-MTBV |
| 2006 | FMD0845 | NEG | 1/320 | NEG | 1/40 | 1/20 | NEG | CARV-MTBV-MURV |
| 2005 | FSL1767 | NEG | NEG | NEG | 1/20 | NEG | NEG | MTBV |
| 2006 | FSL2923 | NEG | NEG | NEG | 1/40 | NEG | NEG | MTBV |
| 2007 | FMD1113 | NEG | NEG | NEG | 1/40 | NEG | NEG | MTBV |
| 2007 | MFI0919 | NEG | NEG | NEG | 1/20 | NEG | NEG | MTBV |
| 2009 | FSL4095 | NEG | NEG | NEG | 1/20 | NEG | NEG | MTBV |
| 1999 | IQT9891 | NEG | NEG | NEG | 1/160 | 1/160 | NEG | MTBV-MURV |
| 1999 | IQU0126 | NEG | NEG | NEG | 1/160 | 1/160 | NEG | MTBV-MURV |
| 2003 | IQD5807 | NEG | NEG | NEG | 1/640 | 1/320 | NEG | MTBV-MURV |
| 2003 | IQD5812 | NEG | NEG | NEG | 1/640 | 1/320 | NEG | MTBV-MURV |
| 2004 | FSL1491 | NEG | NEG | NEG | 1/1280 | 1/1280 | NEG | MTBV-MURV |
| 2004 | IQD8243 | NEG | NEG | NEG | 1/640 | 1/320 | NEG | MTBV-MURV |
| 2004 | IQD8658 | NEG | NEG | NEG | 1/640 | 1/640 | NEG | MTBV-MURV |
| 2005 | IQE1249 | NEG | NEG | NEG | 1/160 | 1/320 | NEG | MTBV-MURV |
| 2006 | IQE3121 | NEG | NEG | NEG | 1/320 | 1/320 | NEG | MTBV-MURV |
| 2006 | IQE4172 | NEG | NEG | NEG | 1/160 | 1/320 | NEG | MTBV-MURV |
| 2006 | SER6020 | NEG | NEG | NEG | 1/160 | 1/160 | NEG | MTBV-MURV |
| 2006 | FLU3627 | NEG | NEG | NEG | 1/320 | 1/320 | NEG | MTBV-MURV |
| 2008 | IQE7620 | NEG | NEG | NEG | 1/640 | 1/640 | NEG | MTBV-MURV |
| 2008 | IQE7743 | NEG | NEG | NEG | 1/640 | 1/640 | NEG | MTBV-MURV |
| 2008 | NFI0798 | NEG | NEG | NEG | 1/160 | 1/160 | NEG | MTBV-MURV |
| 2011 | FPI02026 | NEG | NEG | NEG | 1/320 | 1/160 | NEG | MTBV-MURV |
| 2011 | FPI02271 | NEG | NEG | NEG | 1/320 | 1/160 | NEG | MTBV-MURV |
| 2011 | FPI02607 | NEG | NEG | 1/640 | NEG | NEG | NEG | ITQV (PE00036) |
| 2007 | FMD1043 | NEG | NEG | 1/320 | NEG | NEG | 1/20 | ITQV (PE00036)-ORIV |
| 1955 | APEUV BeAn848 | 1/320 | NEG | NEG | NEG | NEG | NEG | APEUV |
| 1956 | CARV BeAn3994 | NEG | 1/80 | NEG | NEG | NEG | NEG | CARV |
| 1996 | ITQV (PE00036) | NEG | NEG | 1/320 | NEG | NEG | NEG | ITQV (PE00036) |
| 1954 | MTBV BeAn15 | NEG | 1/20 | 1/20 | 1/2560 | 1/160 | NEG | MTBV-MURV |
| 1955 | MURV BeAn974 | NEG | NEG | NEG | 1/320 | 1/320 | NEG | MTBV-MURV |
| 1954 | ORIV BeAn17 | NEG | NEG | 1/20 | NEG | NEG | 1/160 | ORIV |
Indicated values are neutralization titers. With pink background are the group of isolates that were neutralized with highest titer by CAR HMAF and also belongs to linage 1 and 2 from clade I in the M segment sequence phylogeny. With orange background are the group of isolates that were neutralized with very low titer with MTBV HMAF and belong to linage 3 from clade I in the M segment sequence phylogeny. With green background are the group of isolates that were neutralized by MTBV- MURV and belongs to clade II in the M segment sequence phylogeny. With yellow background are the isolates that were neutralized with highest titer by ITQV and belongs to clade III in the M segment sequence phylogeny.