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. 2018 Jul 19;13(7):e0201131. doi: 10.1371/journal.pone.0201131

Fig 4. Protein expression of molecules in the insulin signaling pathway and glucose transporters.

Fig 4

Western blot analysis was used to detect phosphorylated phosphatidylinositol-3-kinases (p-PI3K), phosphorylated Akt (p-Akt), total Akt, glucose transporter (Glut) 1, Glut2 and the internal control β-actin (A). Protein expression of p-Akt (B), Glut1 (C) and IRS2 (D). Protein quantification was carried out by densitometric analysis, normalized by the internal control β-actin, and calculated as the percentages of the NG model without any treatment (0 μg/ml). Values are means ± SEM, n = 9 for each group. Values with different lowercase superscript letters indicate significant differences within each model (one-way ANOVA with LSD, P < .05).