Table 1. Proteins differentially represented in SAL and M82 anther proteomes.
Functional classification and regulation of protein abundance are reported.
| HT/CC (fold change) a) |
M82/SAL (fold change) b) |
||||||
|---|---|---|---|---|---|---|---|
| ACCESSION NCBInr | PROTEIN NAME | SOL GENOMICS NETWORK ID GENE ID |
PATHWAY OR GO TERMS | IA I SAL |
IA II M82 |
IA III CC |
IA IV HT |
| ENERGY METABOLISM | |||||||
| XP_004243619.1 | 2,3-bisphosphoglycerate-independent phosphoglycerate mutase | Solyc07g044840.2 101248497 |
Glycolysis I-V(Plant Cytosol) | -3.32 | -2.12 | -2.04 | -1.30 |
| NP_001332774.1 | enolase | Solyc10g085550.1 101247482 |
1.72 | -1.50 | 1.91 | 2.21 | |
| NP_001234080.1 | enolase | Solyc09g009020.2 544068 PGH1, ER28 |
2.38 | 2.44 | -2.35 | -2.30 | |
| XP_004230885.1 | triose phosphate isomerase chloroplastic | Solyc01g111120.2.1101246286 | -2.01 | -1.26 | -3.21 | -2.01 | |
| NP_001316521.1 | phosphoglycerate kinase | Solyc07g066600.2 101254111 |
-2.59 | -1.93 | 1.08 | 1.50 | |
| NP_001266254.2 | glyceraldehyde 3-phosphate dehydrogenase | Solyc05g014470.2 101258368 GAPC2, GAPDH |
1.50 | 2.88 | -1.55 | 1.24 | |
| XP_004230104.1 | probable ribose-5-phosphate isomerase 3, chloroplastic | Solyc01g097460.2 101264255 | Rubisco Shunt | -1.06 | -2.02 | 1.92 | 1.01 |
| NP_001315977.1 | malate dehydrogenase, mitochondrial | Solyc12g014180.1 101258530 mMDH2 |
TCA Cycle Variation III-IV Glyoxylate Cycle |
-2.32c | -1.20 c | -3.94 c | -2.04 c |
| NP_001234001.2 | mitochondrial malate dehydrogenase | Solyc07g062650.2 7782185 mMDH |
-3.41 | -2.14 | -2.01 | -1.62 | |
| XP_004247734.1 | malate dehydrogenase | Solyc09g090140.2 101253131 |
1.71 | 2.05 | -1.03 | 1.17 | |
| NP_001234293.2 | succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial | Solyc06g083790.2 543863 |
5.44 | 3.26 | 1.06 | -1.57 | |
| NP_001233888.1 | fructokinase-2 | Solyc06g073190.2 544022 FRK2 |
Sucrose Degradation III-I UDP-Galactose Biosynthesis |
1.07 | -1.06 | -1.87 | -2.13 |
| XP_010313061.1 | probable fructokinase-5 | Solyc11g042850.1 101261091 |
-2.13 | -2.54 | -4.21 | -5.02 | |
| NP_001234843.2 | acid beta-fructofuranosidase precursor | Solyc03g083910.2 543992 AI Aiv-1, TAI, TIV1 |
2.07 | 1.03 | 2.99 | -1.50 | |
| XP_004239832.1 | UTP—glucose-1-phosphate uridylyltransferase-like | Solyc05g054060.2 101250892 |
-2.23 | 2.08 | -2.70 | 1.72 | |
| XP_004250240.1 | UTP—glucose-1-phosphate uridylyltransferase isoform X1 | Solyc11g011960.1 101248935 |
1.94 | 4.15 | -1.19 | 1.81 | |
| NP_001296789.1 | pyruvate dehydrogenase E1 component subunit alpha, mitochondrial | Solyc05g006520.2 543639 |
Acetyl-Coa Biosynthesis | 4.66 | 1.74 | -1.03 | -2.74 |
| NP_001333533.1 | aldehyde dehydrogenase family 2 member B7d | Solyc05g005700.2 101254485 SlADH2B7d |
Dopamine Degradation Oxidative Ethanol Degradation III-I |
1.91 | 3.21 | 1.01 | 1.69 |
| XP_004232138.1 | alpha-1,4-glucan-protein synthase [UDP-forming] 1-like | Solyc02g089170.2 101251942 |
Cellulose Biosynthesis | -1.64 | 1.01 | -7.07 | -4.25 |
| NP_001296718.1 | beta-D-xylosidase 2 precursor | Solyc01g104950.2 543515 |
Xylan 1,4-Beta-Xylosidase Activity | -2.61 | -1.51 | -1.58 | 1.10 |
| XP_004239981.3 | pectinesterase 1-like | Solyc05g052120.2 101243787 |
Homogalacturonan Degradation | -1.02 | 1.07 | -2.13 | -1.94 |
| XP_004232705.1 | putative lactoylglutathione lyase-like | Solyc02g080630.2 101251054 |
Methylglyoxal Degradation I | -1.69 | -2.01 | 1.19 | -1.01 |
| XP_004244101.1 | dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 2, mitochondrial-like | Solyc07g064800.2 101268590 |
2-Ketoglutarate Dehydrogenase Complex |
1.90 | 4.50 | -1.87 | 1.26 |
| XP_004251263.1 | ATP synthase subunit d, mitochondrial-like | Solyc11g072450.1 101248453 |
ATP Synthesis Coupled Proton Transport | -2.66 | 1.09 | -2.91 | 1.00 |
| NP_001234281.2 | vacuolar H+-ATPase A1 subunit isoform |
Solyc12g055800.1 543861 |
ATP Hydrolysis Coupled Proton Transport | -1.13 | -2.09 | -1.08 | -2.00 |
| YP_009430460.1 | ATP synthase F1 subunit 1 | Solyc11g039980.1 34678293atp1 |
Proton-Transporting Two-Sector Atpase Complex | -3.15 | -3.95 | -1.18 | -1.50 |
| XP_004252616.1 | soluble inorganic pyrophosphatase PPA1 | Solyc08g083370.2 101249338 |
Phosphate-Containing Compound Metabolic Process | -1.92 | 1.03 | -7.15 | -3.61 |
| NP_001296294.1 | Oxygen-evolving enhancer protein 1, chloroplastic | Solyc02g065400.2 544299 PSBO |
Photosynthesis | 1.10 | 1.13 | 2.07 | 2.12 |
| AMINOACID METABOLISM | |||||||
| XP_004240034.1 | glutamine synthetase-like | Solyc05g051250.2 101261030 |
Nitrate Reduction II-VI (Assimilatory) Ammonia Assimilation Cycle II Glutamine Biosynthesis |
1.16 | 1.83 | -13.59 | -8.58 |
| NP_001309987.1 | glutamine synthetase cytosolic isozyme 1–1 | Solyc04g014510.2 543756 gts1 |
1.75 | 2.44 | -1.50 | -1.04 | |
| NP_001310599.1 | glutamine synthetase | Solyc01g080280.2 543998 GS2 |
1.86 | 2.59 | -1.50 | -1.06 | |
| NP_001292722.1 | glutamate dehydrogenase | Solyc10g078550.1 544015 gdh1 |
Glutamate Biosynthesis/ Degradation |
1.62 | 3.74 | -1.78 | 1.30 |
| NP_001233863.1 | 3-dehydroquinate synthase, chloroplastic | Solyc02g083590.2 544273 DHQS |
-2.05 | -2.86 | 1.01 | -1.39 | |
| NP_001316163.1 | N2-acetylornithine deacetylase | Solyc08g076970.2 101268129 NAOD |
Arginine Biosynthesis III Ornithine Biosynthesis |
-1.55 | -2.43 | -1.51 | -1.04 |
| NP_001296305.1 | S-adenosylmethionine synthase 2 | Solyc12g099000.1 101247506 SAM2 |
Phytosiderophore Biosynthesis S-Adenosyl-L-Methionine Cycle/Biosynthesis |
1.56 | 7.21 | -1.94 | 2.38 |
| XP_010312254.1 | S-adenosylmethionine synthase 3-like | Solyc10g083970.1 101245012 |
-1.77 | 1.31 | -3.76 | -1.62 | |
| NP_001234425.1 | S-adenosylmethionine synthetase 1 | Solyc01g101060.2 544155 SAM1 |
3.55 | 6.52 | 1.06 | 1.95 | |
| NP_001234004.1 | S-adenosylmethionine synthetase 3 | Solyc09g008280.1 544302 SAM3 |
1.81 | 5.49 | -1.77 | 1.71 | |
| NP_001296095.1 | Threonine dehydratase biosynthetic, chloroplastic | Solyc09g008670.2 543983 |
Isoleucine Biosynthesis Hypoglycin Biosynthesis |
1.81 c | -2.86 c | 2.17 c | -2.38 c |
| XP_019068856.1 | fumarylacetoacetase | Solyc04g014730.2 101261704 |
Tyrosine Degradation I | 1.87 | 2.61 | 1.31 | 1.82 |
| OTHER METABOLISMS | |||||||
| XP_004248757.1 | caffeoyl-CoA O-methyltransferase 5 | Solyc10g050160.1 101253032 |
Phenylpropanoid Biosynthesis |
-1.80 | -2.05 | 1.17 | 1.03 |
| XP_004230766.1 | caffeoyl-CoA O-methyltransferase 6 | Solyc01g107910.2 101260278 |
-1.64 | -2.48 | 1.59 | 1.05 | |
| NP_001318059.1 | polyphenol oxidase F, chloroplastic | Solyc08g074630.1 101259064 |
Esculetin Biosynthesis | 1.09 | -3.22 | 3.63 | 1.03 |
| NP_001296326.1 | polyphenol oxidase B, chloroplastic; | Solyc08g074680.2 101258774 PPO |
-1.55 | -2.62 | 1.70 | 1.01 | |
| NP_001234782.1 | cytosolic ascorbate peroxidase 1 | Solyc06g005160.2 778223 APX1 |
Ascorbate Glutathione Cycle | 4.86 | 6.50 | 1.06 | 1.50 |
| NP_001234788.2 | cytosolic ascorbate peroxidase 2 | Solyc06g005150.2 778224 APX2 |
3.91 | 3.71 | 1.06 | 1.01 | |
| NP_001233847.1 | carbonic anhydrase | Solyc02g067750.2 100147727 ca3 |
Cyanate Degradation | 1.68 | 5.58 | -1.92 | 1.73 |
| NP_001296993.1 | carbonic anhydrase, 2 1 | Solyc05g005490.2 543802 Ca1 |
4.34 | 7.85 | 1.06 | 1.92 | |
| XP_004234310.1 | gamma carbonic anhydrase-like 2, mitochondrial-like | Solyc03g019720.2 101249243 |
1.62 | -1.83 | 1.50 | -2.06 | |
| XP_004232424.1 | gamma carbonic anhydrase 1, mitochondrial-like | Solyc02g085040.2 101264478 |
-1.17 | 1.13 | 1.61 | -2.14 | |
| NP_001233862.2 | leucine aminopeptidase 1, chloroplastic | Solyc12g010040.1 544017 LAPA1 |
Seed Germination Protein Turnover Wound-Induced Proteolysis I |
-4.62 c) | -2.33 c) | -2.14 c) | -1.08 c) |
| NP_001233884.2 | Leucine aminopeptidase 2, chloroplastic | Solyc00g187050.2 544277 LAPA2 |
1.86 | 4.50 | -1.92 | 1.26 | |
| NUCLETOTIDE METABOLISM | |||||||
| NP_001296740.1 | UMP/CMP kinase 3 | Solyc01g088480.2 101243930 UMP-CMP kinase 3 |
Adenosine Nucleotides De Novo Biosynthesis | -1.16 | -1.52 | -2.46 | -3.21 |
| NP_001316139.1 | adenine phosphoribosyltransferase 1 | Solyc04g077970.2 101260722 |
Salvage Pathways Of Purine Nucleosides II | -1.72 | -2.15 | 1.26 | 1.00 |
|
XP_004249586.1 |
adenosine kinase 2 | Solyc10g086190.1 101251530 |
3.29 | 1.74 | 1.75 | -1.08 | |
| FOLDING, SORTING AND DEGRADATION | |||||||
| NP_001234225.1 | type I small heat shock protein 17.6 kDa isoform | Solyc06g076560.1 543848 |
Unfolded Protein Binding | 7.93 | -1.04 | 7.16 | -1.15 |
| NP_001266213.1 | heat shock protein 70–3 | Solyc04g011440.2 101055596 hsc70.3 |
1.94 | 4.27 | -1.14 | 1.92 | |
| XP_004249331.1 | nascent polypeptide-associated complex subunit alpha-like protein-like | Solyc10g081030.1 101251235 |
1.54 | -2.04 | 4.97 | 1.58 | |
| P49118.1 | 78 kDa glucose-regulated protein homolog | Solyc08g082820.2 543957 BiP/grp78 |
Misfolded Protein Binding | 1.32 | -1.04 | -1.50 | -2.04 |
| XP_004230445.1 | stromal 70 kDa heat shock-related protein, chloroplastic-like | Solyc01g103450.2 101265681 |
Protein Folding | 1.15 c) | -4.89 c) | 1.06 c) | -3.98 c) |
| XP_004251703.1 | 20 kDa chaperonin, chloroplastic | Solyc12g009250.1 101253560 |
Chaperone Binding | 1.16 | -1.16 | 2.01 | 2.00 |
| XP_004247428.1 | uncharacterized protein OsI_027940 | Solyc09g075010.2 101249490 |
4.22 | 1.80 | 1.25 | -1.88 | |
| XP_004228946.1 | ruBisCO large subunit-binding protein subunit beta, chloroplastic | Solyc01g028810.2 101253117 |
Cellular Protein Metabolic Process- Protein Folding | 2.33 | 2.02 | 1.87 | 1.62 |
| XP_004247810.1 | chaperonin CPN60-2, mitochondrial | Solyc09g091180.2 101250927 |
ATP Binding |
2.53 | 3.18 | -1.94 | -1.54 |
| NP_001315608.1 | cell division cycle protein 48 homolog | Solyc06g074980.2 101266129 |
-1.38 | 1.87 | -1.10 | 2.35 | |
| XP_004250958.1 | heat shock cognate 70 kDa protein 2-like | Solyc11g066060.1 101254866 hsp70-2 |
6.79 | 2.81 | 1.95 | -1.24 | |
| XP_004240392.1 | protein disulfide-isomerase-like | Solyc06g005940.2 101262921 |
Protein Disulfide Isomerase Activity | 2.03 | 1.94 | 1.10 | 1.06 |
| XP_004244803.1 | proteasome subunit alpha type-5-like | Solyc08g016510.2 101266742 |
Endopeptidase Activity | -1.83 | -2.36 | 1.14 | 1.07 |
| XP_004244120.1 | ubiquitin thioesterase OTU1 | Solyc07g064590.2 101248409 |
Ubiquitin-Specific Protease Activity | 1.88 | 3.58 | -1.03 | 1.85 |
| XP_004245731.1 | hsp70-Hsp90 organizing protein 2 | Solyc08g079170.2 101245387 |
Protein Kinase Inhibitor Activity | 4.06 | 1.13 | 2.47 | -1.50 |
| NP_001233872.1 | mitochondrial small heat shock protein | Solyc08g078700.2 543507 MTSHP |
8.96 | 3.58 | 5.12 | 2.04 | |
| NP_001234183.1 | plastid lipid associated protein CHRC | Solyc02g081170.2 778336 ChrC |
Protein Binding |
-2.62 c) | -2.21 c) | 2.63 c) | 3.11 c) |
| NP_001234691.2 | wound-inducible carboxypeptidase | Solyc06g083040.2 544223 |
1.21 | -1.85 | -1.70 | -3.82 | |
| XP_015064515.1 | probable mitochondrial-processing peptidase subunit beta | Solyc02g088700.2 107009685 |
Protein Complex Binding |
-2.08 c) | -2.31c) | -2.01c) | -2.24 c) |
| XP_004239065.1 | probable mitochondrial-processing peptidase subunit beta-like | Solyc05g012480.2 101247218 |
-1.77 | -2.10 | 1.10 | 1.07 | |
| OTHER FUNCTIONS | |||||||
| XP_004251245.1 | elongation factor 1-beta 2 | Solyc11g072190.1 101268350 |
Translation Elongation Factor 1 Complex | -1.89 | 1.30 | -5.80 | -1.90 |
| NP_001234503.1 | Eukaryotic translation initiation factor 5A-2 | Solyc07g005560.2 543668 eIF-5A2 |
Protein N-Terminus Binding | 4.24 | 1.18 | 1.76 | -2.03 |
| NP_001317048.1 | actin -41 | Solyc04g011500.2 101260631 actin |
Structural Constituent Of Cytoskeleton | -1.08 | 1.30 | -2.06 | -1.50 |
| XP_004241231.1 | actin-82 | Solyc06g076090.2 101249734 actin-82 |
1.00 | 1.67 | 1.88 | 3.07 | |
| NP_001234231.1 | remorin 1 | Solyc03g025850.2 543593 rem-1 |
DNA Binding | 1.35 | 1.29 | 2.30 | 2.19 |
| NP_001234104.1 | annexin p34 | Solyc04g073990.2 543564 AN34 |
Calcium-Dependent Phospholipid Binding | -1.04 | -1.06 | 2.06 | 2.02 |
| NP_001316365.1 | dehydrin | Solyc04g082200.2 101253585 dhn |
Response To Water | 1.65 | -1.59 | 3.31 | 1.26 |
| NP_001269248.1 | abscicic acid stress ripening protein 4 | Solyc04g071620.2 101246420 Asr4 |
Response To Stress | 2.56 | 1.07 | 8.92 | 3.73 |
| NP_001266269.1 | inducible plastid-lipid associated protein | Solyc07g064600.2 101248695 CHRDi |
Endoribonuclease Activity | 1.72 | 4.67 | -1.89 | 1.53 |
| AFJ93093.1 | proteinase inhibitor II | Solyc03g020060.2 |
Serine-Type Endopeptidase Inhibitor Activity | -1.28 | -1.94 | 2.28 | 1.50 |
| XP_004235415.1 | serine protease inhibitor 5-like | Solyc03g098760.1 101263388 |
Endopeptidase Inhibitor Activity | 1.01 | 2.07 | -5.06 | -2.47 |
| XP_004253396.2 | multicystatin, partial | Solyc00g071180.2 543570 |
Cysteine-Type Endopeptidase Inhibitor Activity Cobalt Ion Binding |
3.21 c) | 2.39 c) | -1.60 c) | -2.16 c) |
| XP_004230497.1 | ankyrin repeat domain-containing protein 2-like | Solyc01g104170.2 101255143 |
Mechanically-Gated Ion Channel Activity | 1.71 | 3.57 | -1.12 | 1.86 |
| NP_001317632.1 | NAD(P)-linked oxidoreductase serfamily protein | Solyc07g043570.2 101252603 |
Oxidation-Reduction Process | -3.54 | -2.13 | -1.30 | 1.27 |
| XP_010312248.1 | peroxiredoxin-2E-2, chloroplastic | Solyc10g083650.1 104644379 |
Oxidoreductase Activity |
6.61 | 5.74 | 1.06 | -1.09 |
| XP_004237119.2 | Web family protein At1g12150-like | Solyc04g015110.2 101255879 |
-1.10 | 1.33 | -2.41 | -1.65 | |
| XP_015069432.1 | pollen allergen Che a 1-like | 107014082 | -1.11 | 1.10 | -2.05 | -1.68 | |
| XP_004243405.2 | seed biotin-containing protein SBP65-like | Solyc07g053360.2 101259649 |
1.15 | -1.21 | 3.40 | 2.45 | |
| XP_004232206.1 | uncharacterized protein At5g39570 | Solyc02g088260.2 101248442 |
4.71 | 2.24 | 4.08 | 1.94 | |
| XP_010324012.1 | stress-response A/B barrel domain-containing protein UP3-like | Solyc07g041490.1 101256396 |
-2.10 | -3.47 | 2.50 | 1.52 | |
| XP_004239074.1 | nodulin-related protein | Solyc01g104780.2 101249727 |
-2.08 | -1.04 | -1.31 | 1.54 | |
a) Changes in protein levels are reported as the ratio between the normalized protein spot volume from Saladette and M82 tomato anthers grown under high temperature and control conditions (VHT/VCC) for up-regulated proteins and as the negative reciprocal values (-VHT/VCC) for down-regulated proteins.
b) Changes in protein levels are reported as the ratio between the normalized protein spot volume from M82 and Saladette tomato anthers grown under the same conditions (VM82/VSAL) for up-regulated proteins and as the negative reciprocal values (- VM82 /VSAL) for down-regulated proteins.
c) Average fold change for proteins contained in more than one spot has been calculated summing the normalized protein spot volume of all the spots containing the same protein and a fold change ≥ 2.0 has been considered significant.