. 2018 Jul 19;13(7):e0201069. doi: 10.1371/journal.pone.0201069

Table 2. Precision and repeatability of qPCR and ddPCR.

			Normalized to cel-miR-39		Normalized to miR-16		Normalized to mean of cel-miR-39 and miR-16
			Mean	CV (%)	Mean	CV (%)	Mean	CV (%)
miR-92a	qPCR	Precision	16.9	31.4	0.16	22.2	1.62	26.5
	qPCR	Repeatability	16.9	6.8	0.16	6.3	1.62	6.0
	ddPCR	Precision	5.8	26.7	0.22	21.6	1.13	23.7
	ddPCR	Repeatability	5.8	13.2	0.22	4.4	1.13	7.8
miR-126	qPCR	Precision	0.29	15.6	0.0028	10.5	0.028	11.1
	qPCR	Repeatability	0.29	5.1	0.0028	4.8	0.028	4.2
	ddPCR	Precision	0.78	12.4	0.029	5.7	0.15	7.0
	ddPCR	Repeatability	0.78	20.1	0.029	13.4	0.15	15.9
miR-16	qPCR	Precision	107	13.2
	qPCR	Repeatability	107	5.2
	ddPCR	Precision	26.5	12.4
	ddPCR	Repeatability	26.5	11.3

A microRNA pool was reverse transcribed in doublets in 15 independent cDNA syntheses on different days. The transcribed cDNAs were assessed in doublets in 15 independent qPCR and ddPCR. The 15 duplicate measurements were used to calculate the precision (inter-assay variation) and repeatability (intra-assay variation) of the two methods for microRNA quantification. The table shows for each method the mean of all 30 measurements and the coefficient of variation (CV).