Fig 3. CTD of Rel_Sau has no impact on hydrolase activity in vivo.

The conditional rel mutant HG001-55 (inducible by IPTG) was complemented with ATc inducible rel constructs (A). Strains were grown during preculture in presence of IPTG (0.5 mM) and were diluted to an initial OD₆₀₀ = 0.1 and growth monitored over time. As control, strain complemented with empty vector was grown with and without IPTG to illustrate the essentially of the Rel hydrolase (B). Rel constructs were grown without IPTG but addition of ATc (0.1 μg/ml) (C).