Fig 7. Influence of the CTD motifs on Rel_Sau interactions.

S. aureus (p)ppGpp⁰ was complemented with native and mutated Rel_Sau. Cell lysates were mixed with magnetic beads, coated with Rel_Sau antibodies and enriched proteins identified by MS. Proteins significantly (t-test difference > 0.05) less abundant using TGS mutated versus native Rel_Sau as bait are shown in the first column. The effects of the DC or ACT mutation on interaction with these proteins are shown in the second and third column, respectively.