Figure 2.

Study design. Animals (n = 47) we weight-matched and randomly assigned to Sham or burn-injured groups. On Day 0, animals either received a burn injury or control Sham (no burn) procedure. After a recovery period, animals were administered with DMSO vehicle or romidepsin injected intraperitoneally for three consecutive days at 9:00 a.m. (Days 3, 5, and 6). These procedures produced five comparator groups: Sham + DMSO, Burn + anti-Pak1 (Day 6), Burn + DMSO (Day 6), Burn + DMSO (Day 10), and Burn + anti-Pak1 (Day 10) (gray boxes). All functional testing was performed in the afternoon (p.m.) at baseline, Days 3, 4, 5, 6, 7, and 10. Terminal procedures for tissue collection were performed immediately following functional testing at endpoint Day 6 or Day 10. DMSO: dimethyl sulfoxide.