Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Caenorhabditis elegans) |
pdf-1 | NA | WBGene00020317 | |
| Gene (C. elegans) | pdfr-1 | NA | WBGene00015735 | |
| Gene (C. elegans) | daf-7 | NA | WBGene00000903 | |
| Gene (C. elegans) | acy-1 | NA | WBGene00000068 | |
| Genetic reagent (C. elegans) |
ksIs2 | PMID: 11677050 | WBTransgene00000788 | pdaf-7::gfp |
| Recombinant DNA reagent |
Moerman Fosmid Library |
Source Bioscience | WRM0629dH07, WRM0627cG01, WRM0641dA07, WRM068aD11 |
|
| Recombinant DNA reagent |
pPD95.75 | Fire Lab C. elegans
Vector Kit |
Addgene plasmid # 1494 | |
| Recombinant DNA reagent |
pCFJ90 | PMID:18953339 | Addgene plasmid # 19327 |
pmyo-2::mCherry, used as co-injection marker |
| Recombinant DNA reagent |
pZH42 | this paper | pdf-1 genomic DNA in pUC19 | |
| Recombinant DNA reagent |
pZH48 | this paper |
ptrx-1::pdfr-1(cDNA, b isoform,
no STOP codon)::F2A::mCherry:: unc-54 3'UTR |
|
| Recombinant DNA reagent |
pZH53 | this paper | ptrx-1::ACY-1(P260S)::unc-54 3'UTR | |
| Recombinant DNA reagent |
pZH58 | this paper |
pdfr-1p(distal, 5 kb)::loxP::pdfr-1
cDNA(B isoform)::loxP::unc-54 3'UTR |
|
| Recombinant DNA reagent |
pZH59 | this paper | ptrx-1::nCre | |
| Recombinant DNA reagent |
pJDM30 | PMID: 25303524 | ptrx-1::daf-7 | |
| Recombinant DNA reagent |
pSF11 | PMID: 23972393 |
ptag-168::nCre, gift of C. Bargmann and S. Flavell |
|
| Commercial assay or kit |
NEBuilder HiFi DNA Assembly Master Mix |
NEB | E2621 | |
| Software, algorithm | GraphPad Prism | GraphPad | RRID:SCR_002798 | |
| Other | Alexa Fluor 647 DHS ester |
Invitrogen/Thermo Fisher Scientific |
A20006 | dye used for conjugation of FISH probes |