Table 1. Oligonucleotides.
Regions complementary to crRNA A26 are italicized and mismatches are shown in bold. crRNA A26 is shown 3’ to 5’ and the 5’ handle is bold. Phosphorothioate linkages are indicated with an asterisk. For substrate A1, Csx1 cleavage sites are in bold.
| crRNA A26 | 3’-GCAACAATTCTTGCTGCAACAATCTTCAACCCATACCAGAAAGUUA |
|---|---|
| Name | Sequence (5’−3’) |
| Target RNA A26 | AGGGUCGUUGUUAAGAACGACGUUGUUAGAAGUUGGGUAUGGUGGAGA |
| 3’-blunt | AGGGUCGUUGUUAAGAACGACGUUGUUAGAAGUUGGGUAUGGU |
| Match | CGUUGUUAAGAACGACGUUGUUAGAAGUUGGGUAUGGUCUUUCAAU |
| 5’-blunt | CGUUGUUAAGAACGACGUUGUUAGAAGUUGGGUAUGGUGGAGA |
| MM0.5 | AGGGUCGUUGUUAAGAACGACGUUGUUAGAAGUUGGGUAUUUUGGAGA |
| MM1 | AGGGUCGUUGUUAAGAACGACGUUGUUAGAAGUUGGGUCGGGUGGAGA |
| MM1a | AGGGUCGUUGUUAAGAACGACGUUGUUAGAAGUUGGGUAGGGUGGAGA |
| MM1b | AGGGUCGUUGUUAAGAACGACGUUGUUAGAAGUUGGGUCUGGUGGAGA |
| MM1.5 | AGGGUCGUUGUUAAGAACGACGUUGUUAGAAGCCGGGUAUGGUGGAGA |
| MM2 | AGGGUCGUUGUUAAGAACGACGUUGUCGGAAGUUGGGUAUGGUGGAGA |
| MM2.5 | AGGGUCGUUGUUAAGAACGACGUUCAUAGAAGUUGGGUAUGGUGGAGA |
| MM4 | AGGGUCGUUGUUAAAGACGACGUUGUUAGAAGUUGGGUAUGGUGGAGA |
| P-thioate A26 | AGGGUCGUUGUUAAGAACGACGUUGU*U*A*GAAGUUGGGU*A*U*GGUGGAGA |
| Substrate (A1) | AGGGUAUUAUUUGUUUGUUUCUUCUAAACUAUAAGCUAGUUCUGGAGA |
| A3 (MazF) | AAAACAUCAG |
| A4 | AAAAACAUCAG |
| A5 | AAAAAACAUCAG |
| A6 | AAAAAAACAUCAG |
| Trap DNA | GTCGTTCTTAACAACGACCCT |
| Target RNA B2 product | AGGGUCGUUGUUAAGAACGACGUUGUU |