Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (H. sapiens, Male) |
H19101 iPSC | 10.1101/gr.224436.117 | ||
| Antibody | Anti-acetyl Histone H3 (Lys27) (mouse monoclonal) |
Wako Chemicals (USA) | 306–34849 | H3K27ac ChIP-seq |
| Antibody | Anti-cardiac troponin T (mouse monoclonal) |
BD Biosciences | 564767 | CM flow cytometry |
| Chemical compound, drug |
ROCK Y-27632 dihydrochloride |
Abcam | ab120129, 10 mg | iPSC tissue culture |
| Chemical compound, drug |
CHIR-99021 trihydrochloride |
Tocris | 4953 | CM differentiation |
| Chemical compound, drug |
Wnt-C59 | Tocris | 5148 | CM differentiation |
| Commercial assay or kit |
TruSeq RNA libarary prep kit V2 |
Illumina | RS-122–2001 | RNA-seq |
| Commercial assay or kit |
NEBNext Multiplex Oligos for Illumina |
NEB | E7335S | Hi-C |
| Commercial assay or kit |
MEGAshortscript T7 Transcription Kit | Thermo Fisher | AM135 | Probe generation |
| Sequence-based reagent |
Primer A | IDT | 5′-CTGGGAATCGCACCAGCGTGT-3′ | Probe generation |
| Sequence-based reagent |
Primer B | IDT | 5′-CGTGGATGAGGAGCCGCAGTG-3′ | Probe generation |
| Sequence-based reagent |
Primer A T7 | IDT | 5’-GGATTCTAATACGACTCACT ATAGGGATCGCACCAGCGTGT-3’ |
Probe generation |
| Sequence-based reagent |
blocking primer P5 | IDT | 1016184 | Hi-C capture |
| Sequence-based reagent |
blocking primer P7 | IDT | 1016186 | Hi-C capture |