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. 2018 Mar 6;32(8):4132–4144. doi: 10.1096/fj.201701090R

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This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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LCs down-regulate MDA5 on skin barrier perturbation and p(I:C) activation on culture. Immunofluorescence double labeling of cryostat sections (TLR3, PKR, RIG-I) as well as epidermal sheets (MDA5) 48 h after culture of indicated skin explant groups. Representative merged figures with Hoechst (H) nuclear staining are shown from 3 donors in each group. Percentages in merged figures represent means ± sem of results from 3 independent experiments; MDA5⁺CD207⁺ LCs among total CD207⁺ LCs are shown. Tape-stripped (Strp) and p(I:C) group revealed not only less MDA5⁺CD207⁺ LCs but also much weaker MDA5 signal in LCs than respective control groups. Scale bars, 50 µm.