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. Author manuscript; available in PMC: 2019 Jan 4.
Published in final edited form as: Nature. 2018 Jul 4;559(7714):356–362. doi: 10.1038/s41586-018-0288-7

Extended Data Fig. 4. Novel artery markers identified in scRNA-seq data.

Extended Data Fig. 4

(a) e12.5, e14.5, and adult coronary cell rPCA plots with genes highly enriched or specific to the arterial area during development. Each dot is an individual cell, and gene expression levels are indicated by the color spectrum as shown in Fig. 1d, which reflects log10 of counts per million. Red/bolded genes are also enriched in adult artery cells. (b) Fluorescence in situ hybridization (RNAscope) for Slc45a4, which is expressed (arrowheads) in vessels positive for the arterial marker, Cx40, but not Cx40-negative capillaries (arrows). (c) Slc45a4 expression in pre-artery cells derived from the SV lineage (ApjCreER lineage labeled)(arrowheads). (d) Genes enriched in, but not specific to, arterial cells at e12.5 and 14.5. Genes in bold red are arterial specific in both the developing and adult heart. In both a and d: For PCA plots n=415 cells (upper panel, e12.5); n=347 cells (middle panel, e14.5); n=445 cells (lower panel, adult). For bar graphs E12.5, Art, n=20 cells; CV, n=277 cells; SV, n=118 cells; E14.5, Art, n=70 cells; CV, n=454 cells; SV, n=144 cells. Center and error bars are mean ± s.e.m. of log cpm expression values. Dots represent individual cells.

Art, arterial; CV, coronary vessel plexus; SVc, sinus venosus-coronary; V, vein.

Scale bars: 100 μm.