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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Pain. 2018 Aug;159(8):1484–1493. doi: 10.1097/j.pain.0000000000001229

Figure 1. Dorsal root-stimulation induces wind-up in 18% of lamina I SPB neurons.

Figure 1

A–B. Photograph (A) and schematic (B) of recording set up in whole spinal cord preparation. Whole-cell patch clamp recording was made from the lamina I SPB neurons. C. Infrared (IR) and fluorescent image of a lamina I SPB neuron that is labeled with DiI. D. Example traces of wind-up and no wind-up in response to 2 Hz dorsal root stimulation. E. Wind-up is observed in a subset of lamina I SPB neurons in response to 2 Hz root stimulation. F. Pie chart illustrating fraction of lamina I SPB neurons that show wind-up. G. Treatment with the NMDA antagonist APV (50 μM) significantly reduced wind-up in lamina I SPB neurons in response to 2 Hz root stimulation, which recovered upon wash. Data are mean ± SEM (n = 5 cells, paired; asterisks indicate significantly different than control, ** p < 0.01, *** p < 0.001, Two-way ANOVA followed by Dunnett’s multiple comparison test).