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. Author manuscript; available in PMC: 2018 Jul 20.
Published in final edited form as: Nat Microbiol. 2016 Feb 22;1:16011. doi: 10.1038/nmicrobiol.2016.11

Figure 4. HIV-1 replication and RNA nuclear export are perturbed by methylation of A7883 in the RRE bulge region.

Figure 4.

a, WT or LAI HIV-1 mutant constructs, U7871C + A7877G (Mut1), A7883G (Mut2), and the combination of both (Mut3), were assessed for replication in 293T cells under METTL3/METTL14 or AlkBH5 knock-down conditions. Results are the mean ± s.e.m. of three independent experimentsand P values were calculated by unpaired two-tailed Student’s t-test. b, Nuclear export of viral RNA was analysed by cytoplasmic and nuclear fractionation in 293T cells transfected with WT or mutant LAI constructs described in a. Viral RNA distribution in the two compartments is relative to the viral RNA content from total cellular RNA. Results are the mean ± s.e.m. of three independent experiments. P values were calculated by unpaired two-tailed Student’s t-test.