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. 2018 Jul 19;8:10929. doi: 10.1038/s41598-018-29086-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Overall pipeline to detect age-associated splicing events. (B) Seven types of alternative splicing events before and after splicing. Blue rectangles represent constitutive portion of the exons. Purple and beige rectangles represent the alternatively used portion of the exons. Solid lines represent introns. Dashed lines connect the ends of alternatively spliced out portions of the gene. The splicing event structures before splicing and the generated isoform structures after splicing are shown on left and right side respectively.