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. 2018 Jul 19;8:10955. doi: 10.1038/s41598-018-29339-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of pluripotent genes, In vitro and in vivo differentiation and the of iPSCs generated by EGC extract and OSKM combination. (A-a) Morphology of EG-4F-iPSCs P15. (A-b) Alkaline phosphatase-positive colonies and (A-c) normal karyotype (20pairs). (B) Quantitative PCR analysis of pluripotent markers Oct4, Nanog, Rex1 and Sox2 in EG-4F-iPSCs. Error bars indicate standard error of the mean (n = 3). Results were normalized to GAPDH expression. *P < 0.01, versus other cells. (C-a) Embryoid body formation by suspension culture. (C-b) Teratomas. (C-c) RT-PCR analysis of germ layer markers. The grouping blots of germ layer markers were cropped from different parts of the same gel. The grouping blots of gapdh were cropped from different parts of the same gel. (D) Hematoxylin and eosin staining of teratomas. Scale bar = 50 µm. (E) Immunofluorescence staining of pluripotent genes.