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. 2018 Jul 19;8:10957. doi: 10.1038/s41598-018-29069-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DCS and LFS stimulation alters the expression profile of NR2A, NR2B, BDNF and RAB3A. (A) Relative quantity (RQ to Control NoStim) of gene expression change in response to LFS, DCS and LFS/DCS stimulation without toxicity at week 3. (B) Relative quantity (RQ to Control NoStim) of gene expression change following L-glutamate toxicity at week 3 (L-glutamate NoStim). (C) Relative quantity (RQ to L-glutamate NoStim) for gene expression change for LFS, DCS and LFS/DCS stimulation following L-glutamate toxicity at week 3. Data is shown as mean +/−SEM. *, ** and *** indicate p < 0.05, p < 0.01 and p < 0.001, respectively, on two-sample student t-test between test and control samples (n = 6 repeats per group). Light and dark gray dashed lines mark 1- and 2 fold change in expression. Bar plots in order from left to right represent NR2A: NMDA receptor sub unit 2A, NR2B: NMDA receptor sub unit 2B, BDNF: brain-derived neurotrophic factor, RAB3A: RAS-related protein rab3.