Fig. 2.

In vitro analyses of the activity of AsqI against 5. Detailed reaction conditions are described in the Methods section. a In vitro assays of AsqI with 5 as a substrate. HPLC profile of the reaction mixture containing (i) heat-inactivated AsqI with 5 as a negative control; (ii) active AsqI with 5, showing the formation of 6 after 5 min of incubation; (iii) active AsqI with 5, showing a complete conversion of 5 to 6 after 30 min of incubation; (iv) the authentic reference of 5; and (v) the authentic reference of 6. Traces were monitored at 280 nm. b In vitro assays of AsqI with 5 and 8. HPLC profiles of the reaction mixture containing (i) active AsqI with 5 and 8, showing the formation of 6 and 9 after 30 min of incubation; (ii) AsqI and 8 without 5 after 30 min of incubation as a negative control; (iii) 5 and 8 incubated for 30 min without AsqI as a negative control; and (iv) the authentic reference of 9. Traces were monitored at 244 nm. These peaks denoted by asterisks are derived from 8