Fig. 3.

Myeloid cells have an essential role in the induction of fibrosis post-thermal injury. a Mice treated with liposomal clodronate compared with control treated group. Trichrome staining shows less fibrosis around portal venules post-thermal injury when myeloid cells are depleted; n = 9, scale bar 50 μm. b Immunohistochemistry shows a highly effective ablation of F4/80+ cells in liposomal clodronate-treated group in comparison to the control group; n = 9, scale bar 50 μm. c There is a global ablation of F4/80+ cells in the liver of mice treated with liposomal clodronate, scale bar 100 μm. d In contrast to earlier findings, without F4/80+ cells, there is an increase in desmin+ cells post-thermal injury; n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001