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. 2018 Jul 19;9:2827. doi: 10.1038/s41467-018-05154-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — TEN1 loss causes modest G-overhang elongation. G-overhang abundance was analyzed by in-gel hybridization in the indicated cell lines following various times of tamoxifen (TAM) treatment. a, b TEN1 or CTC1 conditional cells. c d TEN1 conditional cells overexpressing exogenous TEN1 or STN1. e f TEN1 conditional cells with/without hTERT overexpression. a, c, e Gels showing hybridization of TAA(C₃TA₂)₃ probe to genomic DNA under native and denaturing conditions. b, d, f Quantification of relative G-overhang abundance. Overhang abundance in TEN1^−/−, CTC1^−/−, and TEN1^−/−-hTERT cells was normalized to that of TEN1^F/F, CTC1^F/F, or TEN1^F/F-hTERT cells respectively (i.e., t = 0). Error bars indicate mean ± SEM, n = 3 independent experiments