Fig. 4.

CTC1-STN1 localizes to telomeres in the absence of TEN1. a–e ChIP showing exogenous FLAG-STN1, FLAG-CTC1, or FLAG-TEN1 localization at telomeres after TEN1 or CTC1 removal. a Representative slot blots to quantify FLAG-STN1, FLAG-CTC1, or FLAG-TEN1 localization to telomeres. Cells were grown with/without tamoxifen for 7 days. b–e Quantification of ChIP data for FLAG-STN1 (b) or FLAG-CTC1 (c) in TEN1 conditional cells, FLAG-STN1 (d) or FLAG-TEN1 (e) in CTC1 conditional cells. Alu probe served as a negative control. f–g ChIP analysis showing the CTC1 1196Δ7 mutant localizes to telomeres. CTC1^−/− cells expressed FLAG-CTC1 WT or FLAG-CTC1Δ7 and were grown with tamoxifen for 7 days. f Representative slot blots. g Quantification of ChIP data. N = ≥3 independent experiments, Error bars indicate mean ± SEM. *P < 0.05; **P < 0.01; NS not significant