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. 2018 Jul 19;8:10902. doi: 10.1038/s41598-018-27643-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MIRAT is expressed in the cytoplasm of melanoma cells resistant to MAPK inhibitors. (a) Bioinformatic pipeline of RNAseq data: the comparison of resistant clones with their parental counterparts applying acute and chronic treatment filters led to the identification of one coding gene (SPOCK1) and one lncRNA (MIRAT) differently upregulated in resistant clones. Expression of MIRAT in melanoma cell lines and tissue samples was determined by PCR and RT-qPCR analyses. (b) Bar graph showing FPKM values of MIRAT in parental and corresponding resistant cell lines. (c) Bar graph displaying MIRAT expression relative to GAPDH determined by RT-qPCR analyses and real time SYBR Green amplification. (d) PCR and RT-qPCR analyses of MIRAT were performed in subcellular cytosolic RNA extracts of D04 and D04RM cells. The expression of MIRAT is displayed as relative values to GAPDH. (e) Bar graph representing MIRAT expression relative to GAPDH in 19 melanoma patients.