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. 2018 Jul 19;8:10902. doi: 10.1038/s41598-018-27643-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MIRAT modulates MAPK signaling through IQGAP1 binding. (a) Ectopic expression of two MIRAT isoforms in D04 parental cells 48 hours post-transfection was determined by PCR (top) and RT-qPCR analysis (bottom) normalized to GAPDH housekeeping gene. (b) Immunoblot showing that MIRAT isoform 2 overexpression results in higher p-ERK levels in D04 cells after MEK inhibition with trametinib compared to respective controls. (c) Bar graph representing MIRAT relative expression in RNA-protein extract of IQGAP1 pull down. MIRAT was found enriched in the extract of DO4-RM and not in the negative control (IgG mouse). (d) Immunoblot showing that silencing of MIRAT and IQGAP1 by esiRNA results in lower p-ERK levels in MM415RM and D04RM trametinib-resistant cells.